era [2019-07-04 13:04:56]

GTP-binding protein, involved in the assembly of the 30S ribosomal subunit

Locus: BSU25290

Isoelectric point: 8.38

Molecular weight: 33.92 kDa

Protein length: 301 aa

Gene length: 906 bp

Function: ribosome assembly

Product: GTP-binding protein

Essential: yes

Synonyms: bex,yqfH

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Ribosome assembly
• Information processing → Targets of second messengers → Targets of (p)ppGpp
• Groups of genes → Essential genes
• Groups of genes → GTP-binding proteins

Gene

Coordinates: 2,610,041 → 2,610,946

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• Binds and hydrolyzes GTP and readily exchanges GDP for GTP

Protein family

• Era subfamily (according to Swiss-Prot) Era/Obg family

Paralogous protein(s)

• YsxC, RbgA, ThdF, Obg, YqeH, YnbA, YphC, YyaF

Effectors of protein activity

• binds ppGpp PubMed, ppGpp may inhibit activity PubMed

Structure

• 3IEV (Era from Aquifex aeolicus, 50% identity/ 77% similarity, complex with the 3' end of 16S rRNA) PubMed

Expression and Regulation

Operons

• Genes: cdd-era-yqzL-recO
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed, in SigB regulon

Biological materials

Expression vectors

• for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP837, available in Stülke lab
• for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP845, available in Stülke lab

Labs working on this gene/protein

• Naotake Ogasawara, Nara, Japan

References

Reviews

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Original publications

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